Journal: Cell chemical biology

Article Title: Synthetic fluorogenic peptides reveal dynamic substrate specificity of depalmitoylases

doi: 10.1016/j.chembiol.2018.10.005

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Cat# sc-221708 CAS# 86855–26-7 phenylmethylsulfonyl fluoride (PMSF) Sigma Aldrich Cat# P7626 CAS#329–98-6 4-nitropehyl octanoate (4-NPO) Sigma Aldrich Cat#21742–1G-F CAS#1956–10-1 Quenched substrate for thioesterases QStE This paper N/A Quenched substrate for esterases QSE This paper N/A Critical Commercial Assays BCA protein assay kit Pierce Cat# 23225 Experimental Models: Cell Lines Human: ovary adenocarcinoma cell line SKOV3 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-77 Human: prostate adenocarcinoma cell line PC-3 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC CRL-1435 Human: prostate carcinoma cell line LnCaP ( Child et al., 2013 ; Garland et al., 2018 ) ATCC CRL-1740 Human: ovary adenocarcinoma cell line OVCAR-3 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-161 Human: breast adenocarcinoma cell line MDA-MB-231 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-26 Human: breast adenocarcinoma cell line MCF7 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-22 Experimental Models: Organisms/Strains T. gondii strain ΔKu80 ( Child et al., 2013 ) N/A T. gondii strain ΔKu80Δppt1 ( Child et al., 2013 ) N/A Mouse: C57BL/6 MMTV-PyMT strain B6.FVB- Tg(MMTV-PyVT)634Mul/LellJ) Jackson Laboratories 022974 Other Syro II automated parallel peptide synthesizer Biotage N/A HPLC 1260 Infinity System Agilent Technologies N/A Finnigan LTQ mass detector Thermo Fischer Sci.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Mass Spectrometry

Journal: Molecules

Article Title: Evaluation of the Anticancer and Biological Activities of Istaroxime via Ex Vivo Analyses, Molecular Docking and Conceptual Density Functional Theory Computations

doi: 10.3390/molecules28227458

Figure Lengend Snippet: The effect of IST on cancer cell proliferation. MCF7, A549, and PC3 cell lines were treated with IST at the indicated concentrations for 72 h. A MTT test was performed to determine the cytotoxic effect of IST. For each panel, the group of cells that were not administered to IST was considered to exhibit 100% proliferation. Each condition was studied in 5 replicates. Mean ± std.

Article Snippet: A549 (CCL-185) and PC3 (CRL-1435) cell lines were obtained from ATCC.

Techniques: